Behavioral Ecology Blog

The goal is this step is to ligate double stranded linkers to each end of the DNA fragments you created with restriction enzymes. The linkers provide PCR primer binding sites for subsequent steps.. It is a pretty cool idea!
1st step is to make the double stranded linkers.. This is easy. Simple mix equimolar [...]

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Tuesday 9/18/07
Step 2 of the protocol calls for a restriction enzyme digest of genomic DNA. The idea is to chop up DNA into pieces small enough so that they can be easily cloned, but large enough to have a good chance of having a microsatellite AND enough flanking sequence to allow for primer development.
The authors [...]

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