Tuesday 9/18/07
Step 2 of the protocol calls for a restriction enzyme digest of genomic DNA. The idea is to chop up DNA into pieces small enough so that they can be easily cloned, but large enough to have a good chance of having a microsatellite AND enough flanking sequence to allow for primer development.
The authors [...]
Entries Tagged as 'lab work'
Microsatellite isolation step 2
September 19th, 2007 · No Comments
Tags: biology · lab work · microsatellite isolation · molecular biology
Microsatellite Isolation and frustration with the current state of the internet
September 18th, 2007 · 3 Comments
Monday I started the long process of isolating microsatellites de novo for 5 mammal species…. I’ll plan on posting a step by step account of how things are going, so interested people and family can stay up to date. But in addition to this, I hope that this series of posts will serve to [...]
